Boston

FutureLabCamp2.5 is taking place at the t=0 festival at MIT in Boston September 16-18th 2011.

where:
the Z center/Johnson in the “ice rink” (it’s near the student center).

t=0 schedule:
The t=0 official schedule: t0.mit.edu/wp-content/uploads/2011/09/t0-Schedule.pdf.

FLC sub-schedule:
6pm Friday night we’ll participate in startup-lab brainstorming.
11am Saturday, we’ll run through genomic extraction, PCR, and analysis w/ electrophoresis and then invite participants to hack on that toolchain until Sunday evening.
5pm Sunday demotime for all hacks!

participate in FLC2.5!



Boston updates:

Announcing FLC2.5 at t=0

Invitation

Biohackers,

A quick and short-timed announcement: FutureLabCamp2.5 is going to occur this weekend at the “t=0” hackathon / conference / festival at MIT. We’ll have a little brainstorming on Friday night and hack all day Saturday and Sunday.

We’re starting things off with a workshop on basic molecular biology on Saturday morning. We’ll walk everyone through genomic DNA extraction, PCR, and gel electrophoresis with traditional equipment… then we’ll ask the participants to prototype a way to do part of the process better, cheaper, faster, or more DIY.

If any of you would like to volunteer to help, please email me (mac@futurelabcamp.com) – I have a limited number of registration slots for DIYbioers and FLC alumni :)

Cheers,
Mac

About

FutureLabCamp 2.5 – Hacking the molecular biology toolchain
Biotechnology is expensive and complicated. It shouldn’t be. We’re bringing a conventional molecular biology toolchain (equipment and reagents) for genomic DNA extraction, DNA amplification with PCR, and DNA length analysis with gel electrophoresis. We’ll run a hands-on workshop Saturday morning to show you how it all works, and then ask you to hack on it and make any part of the process cheaper, easier, faster, better, more DIY-friendly, etc. Folks from the local diybio scene will be present. More info: futurelabcamp.com/boston.

Location and Schedule

where:
the Z center/Johnson in the “ice rink” (it’s near the student center).

t=0 schedule:
The t=0 official schedule: t0.mit.edu/wp-content/uploads/2011/09/t0-Schedule.pdf.

FLC sub-schedule:
6pm Friday night we’ll participate in startup-lab brainstorming.
11am Saturday, we’ll run through genomic extraction, PCR, and analysis w/ electrophoresis and then invite participants to hack on that toolchain until Sunday evening.
5pm Sunday demotime for all hacks!


Boston: FLC2 schedule

FutureLabCamp Boston was held on May 27th-30th (Memorial Day weekend) at Templeman Automation in Somerville, Massachusetts.

Weekend Projects:

Photos of the event are available on Flickr, and the presentations are available on scribd. We’ll even have videos up on vimeo someday :) .

See you next time!


Schedule

Friday:
6:30 PM – Drinks & Snacks at the venue; informal chatting
7:30 PM – Lighting Talks Begin!
8:30 PM – Structured Brainstorming & team formation
9:30 PM – Done for the night

Saturday:
10:30 AM – Coffee n Bagels; team formation
11:00 AM – Hacking Begins

Sunday:
4:00 PM – Time to work on demo / presentation
6:00 PM – DEMOTIME (5 min per team)
7:00 PM – FLC-BOSTON is concluded


Boston: Flatbed Gel Electrophoresis Scanner

Who: Mac Cowell (@100ideas) and Sean Jeffries

What: Retrofitting a flatbed scanner for gel electrophoresis illumination for GelRed or GelGreen dyes


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Sean and I bought a Canon FB 620P flatbed scanner from a Craigslist-Boston listing for $20 on Saturday. We took it apart, inspected the illumination and imaging optics, and tried imaging a GelRed-stained gel with it while transilluminating the gel with UV from an older fotodyne UV transilluminator. Unfortunately, all attempts to use UV light from the fotodyne box resulted in saturated images. This probably results from infrared radiation coming from the fotodyne box, which the scanner’s linear CCD does not filter.

We also tried using a $45 hand-held 365nm “mini” UV lamp from UVP. Fluorescence was barely visible with an 8-second exposure with a conventional SLR camera. No fluorescence was visible with the flatbed scanner. Interestingly, the scanner did not display the same saturated signal as it did with the fotodyne illuminator, perhaps indicating the UVP mini lamp has a reduced infrared output.

Sean and I are continuing to work on the gel scanner project. We modified the canon scanner to only output blue light from it’s integrated RGB LED light source, and we’ve purchased a variety of low-cost filters from theater supply companies, all hopefully capable of filtering blue light illumination from the orange emission light from fluorescing GelGreen. Here’s our list of filters we’re planning to test.

We also purchased another $20 scanner from craigslist, an epson 1200u perfection g752a. Interestingly, the imaging system in the epson scanner involves a much smaller linear CCD and an array of mirrors to project the scan-line onto it through a magnifying lens. The canon scanner we bought first is much simpler in it’s design, and is based on a single RGB led as a light source, a clever linear waveguide made of plastic designed to scatter the LED’s light uniformly along the scan line, and some kind of mysterious integrated linear imaging system, probably a CCD.

We’ll provide some updates next week with the results of our ongoing tests.


Boston: Peristalsis! YEAH!

Who: MiHi (@mihi_tr) and Matt (@interzoneboy)

What: 3D-printed peristaltic pump mechanism

Brief: http://peristalsis.tentacleriot.eu/ – PUMP Y U NOT TRANSPORT MOAR LIQUID?


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Peristalsis is most commonly used to refer to rhythmic smooth-muscle contractions that provide motility in the digestive tract. This type of rhythmic contraction is the basis of a class of pumps — peristaltic pumps — that move fluids through tubes without direct contact with pump components. This is a particular advantage in biological/medical applications where sterility is of utmost importance, as the pumped material need not contact any surface except the interior of the tube.

The peristaltic pump demonstrated uses a rotating hexagonal bearing sandwich (om nom nom!) in which the tube is pinched by the bearings against a concave thing. Three bearings contact the tube at any given time, so the fluid in the tube is moved as the rollers move. This creates lower pressure at the inlet, and higher pressure at the outlet, so the fluid moves.

    Bill of Materials

  • Makerbot stepper motor (NEMA-14)
  • Arduino UNO
  • L297 stepper motor controller
  • L298 stepper motor driver
  • handful o’ diodes
  • buncha resistors et al
  • potentiometer
  • stuff for 12v and 5v protoboard power supply
  • one small wooden board
  • tons of printed parts! See thingiverse
  • skateboard bearings
  • misc nuts and bolts to taste
  • git clone http://git.tentacleriot.eu/peristalsis

Boston: Pocket Topologies

Who: Chris Woebken (woebken.net)

What: 3D-printed mini topological maps embodying biological + geospatial datasets



Boston: Biohackturing & applied biohacking brainstorming

Who: Randy Anway, Deb Cha, Ian Hawkins, Lindsey Mysse, & co.

What: Brainstorming fun, introductory applications of introductory biotechnology.

The group spent the weekend brainstorming potential practical biotechnology applications for beginners. Randy Anway developed a conceptual biotech education framework centered on manipulating carrots that he calls Biohackturing, and Deb Cha et al presented 4 separate concepts of applied biotechnology projects.

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BioHackTuring presentation

BioHackTuring

Biohackturing is a new concept that combines engages whole-brain thinking and social learning. Following biomimetic ‘life principles’ the program is structured to be economically, environmentally, and socially sustainable.

The core program consists of a set of related learning activities that are inexpensive to implement, fun to do, and help build capacity for the future while taking care in the present. Under the guidance of program mentors, existing activities may be modified or adapted and new activities may be added into the program framework.

Activities include a range of ‘action research’ areas, from food preparation to electronics. Program modules involve ‘recipes’ that require various degrees of preparation and effort, but once completed provide a ‘platform’ for future activities. An activity platform may entail a specific skill-set, equipment, some kind of product, or combination of these.

The support program consists of developmental opportunities for mentors and advanced participants, provided through local sponsorship. While the program structure is flexible and not limited with regard to age or educational attainment, specific activities and events will be most effective when tailored to the needs of specific participant groups under the guidance of local sponsors and mentors.

A support network is starting up at biohackturing.com.

DebCha applied biotech concept brainstorming

I) BioBear: The Living Nightlight

BioBear is a cuddly bear that glows blue when squeezed or shaken. The light will gradually fade when it’s left alone. Don’t forget to feed your bear!
Isolation of bioluminescent bacteria from squid:

 


 

II) Grow your own paper

Make your own paper from a vat of tea!  Kombucha is sweet tea fermented with a mixture of yeast and bacteria. The bacteria from a mat of cellulose fibres. To make paper, the fibres can be cleaned, pulverized, and formed into sheets.

Resources:

 


 

III) Make it lemony-fresh!

Grow Bacillus subtilis (as found in yogourt) that produces limonene, which is what makes oranges smell citrus-y.

Transfect B. subtilis with limonene synthase:

 


 

IV) Glow-in-the-dark, water-repellant biofilms

Transfecting RFP or luciferase into B. subtilis to make glow-in-the-dark, water-repellent biofilms

resources:


Brainstorming Session Images

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Biohackturing LinkFest

Cloning carrots

digital water testing meter

hydroponics

toxic fertilizer database

Plant Nutrients

Carrot composition

Make stuff


Boston participants


Quite an exciting group of participants have self-assembled for FutureLabCamp Boston! Can’t wait to see the collaborations bloom. Here’re some descriptions of the participants:

I hack makerbots and am working on building a Reprap mendel. I am a senior at Tufts University


About to start working with Gingko Bioworks on BioBrick-related projects, but I could always use more bio experience. I’m a Somerville resident with DIY leanings, intelligence, and drive.


No doubt that the next 50 years are the years of the life sciences revolution. My passion and role at IDEO is to enable and enhance this revolution through design.


Sounds like a lot of fun, and I want to get a crash course in hacking all sorts of stuff


I am currently a Ph.D. student at BU studying Biomedical Engineering, I recently heard about diyBio groups and would like to start participating.


I’m a biomimicry specialist, who regularly plays at the interface between biology, design, computation, and engineering all over the world to help solve sustainability problems. I just started on my tweet-a-watt project last week, and have plans for hacking together outdoor sensors with long term plans of creating micro-energy sensor networks that can monitor ecological or urban ecology metrics.


I’m an artist and a hacker who wants to spend a manic weekend prototyping a strange new future. I would like to do FLC-Boston because I want to help create a strange new world. I would be offering my design/illustration/film making skills to a team. I will stop by and give a lightning talk on Friday about bio-design automation tools, hardware, and methodologies.


“As a recent graduate with a degree in Biology, work-experience in clean-tech, studies in architecture, and a minor in art, I have become hell-bent on creating my own path that combines my eclectic studies. I have been hacking things since I was old enough to hold a screwdriver and currently have notebooks of ideas piled around my room. I would like to participate in FLC Boston because the chance to sit down with like-minded individuals and sprint from idea to prototype excites the heck out of me.


“NY event was so great that I cannot miss the edition happening in the city where I live. I’d really like to continue with the “”electronic”" thermocycler initiated in NY.


I build small computers for scientists (and artists).


Learn a bit more about hardware hacking for biology. Try something new. Build a turbidometer?


to make something glow probably


To share my bioengineering/biomaterials knowledge and to create something new.


I do biology, comp sci, and hardware mangling, and want to have fun and meet like minded people. <3


I am a computer engineering student and an officer of BUILDS, a hackerspace at Boston University. I am particularly interested in areas combing high-tech / engineering with biology (specifically humans).


I’m a hacker who was educated as MD. I want to live in a world where biotech is in the hands of hackers http://utop.li/83.


“This seems like too much fun to pass up. It would be great to get a team together to hack a prototype ‘microbial mineral precipitate’ or ‘hydrophobic biofilm’. The trick might to be to find evidence of this happening in or on an actual material in the field (such as concrete or brick) – by collecting likely samples and identifying microbes present. Probably would need some swabs, collection bags, scrapers, petri dishes & growing medium for something like soil bacteria such as bacillus pasteurii. I pulled a few research papers off the web for background.”


I’m about to do an intense 8-week bootcamp in mobile app development. As an activist/coder/”social entrepreneur” this is exactly the sort of thing I want to be doing for fun.


Boston Schedule

Friday:
6:30 PM – Drinks & Snacks at the venue; informal chatting
7:30 PM – Lighting Talks Begin!
8:30 PM – Structured Brainstorming & team formation
9:30 PM – Done for the night

Saturday:
10:30 AM – Coffee n Bagels; team formation
11:00 AM – Hacking Begins

Sunday:
4:00 PM – Time to work on demo / presentation
6:00 PM – DEMOTIME (5 min per team)
7:00 PM – FLC-BOSTON is concluded


Boston Venue

Somerville-based Engineering Firm Templeman Automation is graciously hosting FutureLabCamp Boston.

TA is located at 21 Properzi Way, Somerville, MA, which is in the Union Square area and accessible by the 87 bus on the MBTA.

Lost? Call Mac at 231.313.9062

.


View Larger Map


FLC Boston flyer

Well, FLC-Boston is only 28 days away! Here’s a flyer to promote the event (download hi-res PDF here):

Promotional Poster for FutureLabCamp-Boston


Boston Venue: Templeman Automation

The fine engineers at Templeman Automation have offered FLC use of their awesome engineering lab.  They are located in Union Square in Somerville, MA, a short ride from Harvard University.

TA has some really interesting tools for FLCampers to work with, including:

a machine shop, including a diy 3-axis CNC machine


TA has a big main room with 4 work clusters

TA's main work room

a makerbot & a custom in-house multitouch table

The electronics work bench

TA is cool with FLCampers burning the midnight (or 6-am) oil, so pack your sleeping bag.  Parking will be available nearby.